Healing
Growth Factors. 2009 Dec;27(6):448-55.
Bovine colostrum promotes growth and migration of the human keratinocyte HaCaT cell line.
Kovacs D, Cardinali G, Aspite N, Picardo M.
San Gallicano Dermatological Institute, IRCCS, Rome, Italy.
Bovine colostrum represents a rich source of growth factors, which are known to play a central role in wound healing. The aim of our study was to investigate the possible mitogenic and motogenic effects induced by colostrum on human keratinocytes. Cell proliferation evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide test and 5-Bromo-2′-deoxyuridine incorporation revealed that colostrum exerts a growth promoting activity. Scratch assay and immunofluorescence of actin cytoskeleton showed its effectiveness also in inducing cell migration. Furthermore, colostrum treatment increases the levels of tyrosine phosphorylated proteins and the activated forms of the extracellular signal-regulated kinases 1 and 2 and such effects appear to be repressed by the tyrosine kinase inhibitor genistein. Our results indicate that the biological activities of colostrum are specifically mediated by the growth factor-induced activation of tyrosine kinase receptors and underline the relevance of the synergistic action exerted by the growth factors in stimulating keratinocyte proliferation and migration essential for tissue repair.
PMID: 19919532 [PubMed - in process]
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J Med Food. 2009 Aug;12(4):836-45.
Mare’s colostrum globules stimulate fibroblast growth in vitro: a biochemical study.
Zava S, Barello C, Pessione A, Garoffo LP, Fattori P, Montorfano G, Conti A, Giunta C, Pessione E, Berra B, Giuffrida MG.Institute of General Physiology and Biological Chemistry, University of Milan, Milan, Turin, Italy.
The wound repair function of mare’s milk and colostrum was investigated. Mare’s colostrum improved wound healing in vivo; thus fibroblast growth activation by mare’s milk and colostrum was examined. As expected, colostrum was more effective than milk. To establish the biochemical nature of the bioactive molecules involved, colostrum was fractionated into whey, casein, and fat globules, and the efficacy of these fractions on fibroblast proliferation was studied. The fat globule fraction provided the strongest stimulation; its composition was studied and compared with the less-active milk fat globule fraction. The lipid pattern highlighted several differences between mare’s colostrum and milk; in particular, total lipid, linoleic acid, linolenic acid, ganglioside, and glycolipid contents were higher in colostrum. A proteomic investigation revealed some differences between the protein composition of colostrum and milk fat globules. Adipophylin and lactadherin were significantly overexpressed in colostrum fat globules. The role of specific lipids on skin wound repair and that of the epidermal growth factor-like domain, embedded within the lactadherin molecule and probably released in conditions stimulating proteolysis, are discussed.
PMID: 19735185 [PubMed - indexed for MEDLINE]
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The Journal of clinical investigation. 1989 Dec;84(6):1757-61.
Epidermal growth factor enhances renal tubule cell regeneration and repair and accelerates the recovery of renal function in postischemic acute renal failure.
Humes HD, Cieslinski DA, Coimbra TM, Galvao C.
Department of Internal Medicine, Veterans Administration Medical Center, Ann Arbor, Michigan 48105.
Excerpt
The data presented in these current studies demonstrate that exogenously administered EGF can play an important role in the repair and recovery of renal injury after a significant ischemic event. Exogenous EGF produced an accelerated renal tubule epithelial cell regenerative and repair response compared with normal circumstances. This enhanced replicative and repair process led to more rapid relining of the injured renal tubular epithelium and a shortened time to recover renal excretory function. This improvement in renal excretory function with exogenous EGF administration after renal ischemia has also been recently reported in preliminary form from another laboratory (28). EGF has also been demonstrated to be important in repair processes in other tissues. EGF found in saliva has been suggested to accelerate the repair of cutaneous wounds in rodents as they lick the area of injury (29). Topical administration of EGF enhanced the repair of skin injuries…. TGF-alpha also has been shown to accelerate regeneration of the epidermis after partial thickness bums or split-thickness incisions in vivo (33). This report is the first demonstration that EGF may also accelerate the repair process of a visceral organ after an injurious insult.
Abstract
To determine the timing and location of renal cell regeneration after ischemic injury to the kidney and to assess whether exogenous epidermal growth factor (EGF) enhances this regenerative repair process to accelerate recovery of renal function, experiments were undertaken in rats undergoing 30 min of bilateral renal artery clamp ischemia followed by reperfusion for varying time intervals. Renal cell regeneration, as reflected by incorporation of radiolabeled thymidine within the kidney, began between 24 to 48 h and reached a peak at 72 h after renal ischemia. As demonstrated by histoautoradiography, renal thymidine incorporation was essentially confined to tubule cells. Morphometric analysis of histoautoradiograph sections of renal tissue demonstrated that the majority of labeled cells were found in renal cortex, but some labeled cells were also located in the inner stripe of the outer medulla, suggesting that injury to medullary thick ascending limbs also occurs in this ischemic model. Exogenous EGF administration produced increases in renal thymidine incorporation compared with non-treated animals at 24, 48, and 72 h after ischemic injury. This accelerated DNA replicative process was associated with significantly lower peak blood urea nitrogen (BUN) and serum creatinine levels, averaging 63 +/- 20 and 3.1 +/- 0.4 mg/dl in EGF-treated ischemic rats compared with 149 +/- 20 and 5.1 +/- 0.1 mg/dl, respectively, in nontreated ischemic rats, and was also associated with a return to near normal BUN and serum creatinine levels in EGF-treated animals approximately 4 d earlier than that observed in nontreated animals. This report is the first demonstration that EGF accelerates the repair process of a visceral organ after an injurious insult.
PMID: 2592559 [PubMed - indexed for MEDLINE]PMCID: PMC304052